Born in the Republic of San Marino and trained as an engineer, Luca Grassi started his music studies in Bologna. In 1998 he was successful in the Città di Roma competition, where he made his début as Germont in La traviata. In the same year he performed, in Czech, the title rôle in Šarlatán by Pavel Haas at the Wexford Festival.

He has been a regular guest of the Martina Franca Festival, and appeared with Glyndebourne Touring Opera throughout Italy, with Scottish Opera and with the Deutsche Oper in Berlin. He sang Germont under Lorin Maazel at the reopening of La Fenice in Venice, and has also appeared in Japan and Hong Kong.